Discuss the basic principles of next-generation sequencing (NGS) including DNA barcoding and parallel sequencing by synthesis

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Week 5 Quiz – Next generation sequencing technologies I (total RNAseq and whole Genomes)

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Overview:
For this session, we will discuss the basic principles of next-generation sequencing (NGS) including DNA barcoding and parallel sequencing by synthesis. Specific attention will be given to short-read sequencing technology pioneered by companies such as Illumina Inc. The process of library preparation for total RNAseq and whole genome sequencing methods will be discussed. Key factors such as RNA and DNA quality will be reviewed. We will also introduce basic concepts related to bioinformatic analysis of NGS sequencing data from total RNAseq and whole genomes, reviewing publicly available analysis pipelines such as the Broad’s GATK method.
Goals:
Following this session students will be able to:
Understand the basic principles of next generation sequencing
Explain the process of sequencing by synthesis
Describe the difference between total RNAseq and whole genome analysis
Understand the basic principles of bioinformatic analysis of whole genome and whole exome sequence data

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